The Pichia System
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منابع مشابه
P-65: Effective Parameters on the Bovine Follicle Stimulating Hormone Expression in The Pichia Pastoris System
Background: Bovine follicle-stimulating hormone (bFSH) is a heterodimer hormone that consists of a common -subunit which noncovalently associated with the hormone-specific -subunit. During the past 15 years, the methylotrophic yeast Pichia pastoris has become an important host organism for recombinant protein production because it is able to use methanol as a sole carbon and energy source. Th...
متن کاملP-77: Optimization of Ovine FSH Gene Expression in The Pichiapastoris System byRegulating The Culture Conditions
Background: Ovine follicle stimulation hormone (OFSH) is a pituitary glycoprotein and belongs to the family of glycoprotein hormones. This hormone plays a key role in the function of the reproductive system: it is essential for sertoli cell function and spermatogenesis in testis and it stimulates the growth of ovulatory follicles in females. Ovine FSH hormone is a heterodimeric hormone consisti...
متن کاملP-198: Utilization of Pichia Pastoris Secretion System for Expression of Equine Follicle Stimulating Hormone
Background: Equine follicle stimulating hormone (eFSH) is a pituitary heterodimeric glycoprotein consists of noncovalently linked of generic alpha subunit and a hormone specific beta subunit. The molecular weights of the subunits are similar and about 16 KD. In general, FSH plays a key role in controlling vertebrate gonadal functions. In female mammals, ovarian maturation and follicular growth ...
متن کاملP-205: Production of Recombinant Fish FSH Hormone in Pichia Pastoris
Background: Follicle-stimulating hormone (FSH) belongs to the family of glycoprotein hormones that composing alpha and beta subunits with non-covalently bonds. This hormone involve in regulation of the reproductive processes such as gamete generation and follicular growth. Injection of the hormone in most of fish species increases 17 beta-estradiol production by ovarian tissue and also stimulat...
متن کاملExpression of the VP2 gene of classical D78 infectious bursal disease virus in the methylotrophic yeast Pichia pastoris as a secretory protein
Infectious bursal disease virus (IBDV) is the causative agent of Gumboro disease, an infectious disease of global economic importance in poultry. The expression of heterologous proteins in P.pastoris is fast, simple and inexpensive. In this study, VP2 encoding gene of classical D78 IBDV was amplified using reverse transcription (RT) polymerase chain reaction (PCR) and cloned into pPICZαA vector...
متن کاملCloning of fusion (F) protein gene of peste des petits ruminants virus (PPRV) in secretory Pichia pastoris vector
With advent and development of DNA recombinant technology and advantages of p. pastoris expression system, fusion (F) protein of PPRV expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against PPR disease. In this study, F gene of PPRV Nigeria 75/1 strain (1637 bp) was amplified using RT-PCR and purified. It was then cl...
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